The biochemical and growth-associated traits of basil (Ocimum basilicum L.) affected by silver nanoparticles and silver.

BACKGROUND: The biochemical and growth changes resulting from exposure of basil (Ocimum basilicum L.) seedlings to silver nanoparticles and silver were investigated. Over a two-week period, seedlings were exposed to different concentrations (0, 40, and 80 ppm) of silver nanoparticles and silver. RESULTS: Our findings revealed that at concentrations of 40 and 80 ppm, both silver nanoparticles and silver nitrate led to decreased weight, root and shoot length, as well as chlorophyll a and b content. Conversely, these treatments triggered an increase in key biochemical properties, such as total phenols, carotenoids and anthocyanins, with silver nanoparticles showing a more pronounced effect compared to silver nitrate. Moreover, the levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) rose proportionally with treatment concentration, with the nanoparticle treatment exhibiting a more substantial increase. Silver content showed a significant upswing in both roots and leaves as treatment concentrations increased. CONCLUSIONS: Application of varying concentrations of silver nanoparticles and silver nitrate on basil plants resulted in reduced growth and lower chlorophyll content, while simultaneously boosting the production of antioxidant compounds. Notably, anthocyanin, carotenoid, and total phenol increased significantly. However, despite this increase in antioxidant activity, the plant remained unable to fully mitigate the oxidative stress induced by silver and silver nanoparticles.

Light emitting diodes improved the metabolism of rosmarinic acid and amino acids at the transcriptional level in two genotypes of Melissa officinalis L.

In the present study, we used different LEDs to evaluate their effect on metabolic and transcriptional reprogramming of two genotypes (Ilam and Isfahan) of lemon balm grown under narrow-band LED lighting. Lemon balm plants were grown in four incubators equipped with artificial lighting and subjected to four LED lamps [White, Blue, Red, and mixed RB (Red+Blue) (70%:30%)] and in greenhouse conditions for 7weeks. The results showed significant increases in leaf number, pigment and soluble sugar contents, secondary metabolites, and calcium, magnesium, potassium and amino acid contents achieved in growth under mixed RB LEDs. As observed for the content of total phenolics, rosmarinic acid, and amino acids, the expression of genes involved in their production, including TAT , RAS , and DAHPS were also enhanced due to the mixed RB LED lighting. The best condition for both the plant growth and expression of genes was under the mixture of Red+Blue LED lamps. These observations indicate that the increase in secondary metabolites under mixed Red+Blue lights may be due to the increase in primary metabolites synthesis and the increased expression of genes that play an essential role in the production of secondary metabolites.

LED light sources improved the essential oil components and antioxidant activity of two genotypes of lemon balm (Melissa officinalis L.).

BACKGROUND: Nowadays, light-emitting diodes (LEDs) as a new lighting technology, have been emerged as an alternative source of light for plants due to their wavelength specificity, the narrow width of their bands, small size, solid structure, long lifetime, and low heat generation. Here we investigated the effect of different LED light sources on the essential oil components and antioxidant activity of Melissa officinalis. Two genotypes of lemon balm (Ilam and Isfahan) were subjected to four artificial light treatments, including white, red, blue, red + blue LEDs, and greenhouse light as natural lighting. RESULTS: The LED lights significantly increased shoot fresh and dry weights and leaf number in the two genotypes as compared to greenhouse condition. The results showed that the content and composition of essential oil in the two genotypes were variable under different light treatments and the total amount of compounds in the Ilam genotype was higher than the other genotype. The results of analysis of the essential oil by GC/MS indicated that the highest amount of monoterpenes in the genotypes was related to citronellal under red + blue LED lamps (15.3 and 17.2% in Ilam and Isfahan genotypes, respectively) but blue, white, and greenhouse condition had the most effect on sesquiterpenes content in both genotypes. The results showed that the observed variation between the two genotypes in the essentials oil composition was related to the relative percentage of the constituents and not to the appearance or lack of a specific component. Red + blue lighting also provided the highest radical scavenging activity in both genotypes (80.77 and 82.09% for Ilam and Isfahan genotypes, respectively). Based on principal component analyses (PCA), three main groups were identified regarding genotypes and all light treatments. CONCLUSIONS: Overall, results indicated that the essentials oil composition of two genotypes of lemon balm was affected both qualitatively and quantitatively by different LED light sources; hence, LED lights might be used to improve monoterpenes, sesquiterpenes, and antioxidant activity in the selected genotypes.

LED light mediates phenolic accumulation and enhances antioxidant activity in Melissa officinalis L. under drought stress condition.

The popularity of lemon balm in conventional medicine is suggested by the existence of two groups of compounds, namely essential oil and phenylpropanoids pathway derivatives. One of the promising approaches to improve tolerance to drought stress induced oxidative damage in seedlings grown in greenhouses and plant growth chambers is replacing the traditional and high-cost light technologies by recently developed light emitting diodes (LED). In this experiment, we analyzed the role of various LED lights including red (R), blue (B), red (70%) + blue (30%) (RB), and white (W) as well as normal greenhouse light (as control) to stimulate defense mechanisms against drought stress in two genotypes of Melissa officinalis L. The present study demonstrates that pre-treatment with LEDs with high-intensity output for 4 weeks alleviated harmful effects of drought stress in the two genotypes. Under drought stress, RB LED pre-treated plantlets of the two genotypes exhibited the highest relative growth index of shoot and root and total phenolic and anthocyanin content compared to those irradiated with other LEDs and greenhouse lights. The highest amount of malondialdehyde level was detected in R LED exposed plants. In response to drought, LED-exposed plants especially RB light-irradiated plants of the two genotypes maintained significantly higher antioxidant and phenylalanine ammonia-lyase (PAL) enzyme activities and higher expression level of the PAL1 and 4CL-1 genes compared to those irradiated with greenhouse light. We concluded that RB LED light provides a better growth condition and resistance to drought stress for the two genotypes of lemon balm by the highest antioxidant activity and the least amount of damage to the cell membranes. Our data suggest that LED light pre-treatments as moderate stress activate antioxidant systems, enhance the scavenging of ROS and induce drought stress tolerance in the two genotypes of lemon balm plants.

Development of the Farsi Hearing in Noise Test.

Objectives: Develop sentences in Farsi for use in the Hearing In Noise Test (HINT); equalise the difficulty of the sentences; form twelve 20-sentence lists; and determine the reliability and norms for these materials.Design: The same study design as used in the development of HINT in other languages was followed. Sentences were sampled from elementary school reading materials. Sentence difficulty was matched by scaling sentence levels. Equivalent sentence lists were formed. Speech reception thresholds were measured under headphones in quiet and in three noise conditions that differed in the spatial locations of the speech and noise sources. Spatial locations were simulated using head-related transfer functions.Study sample: Subjects were 24 adult native speakers of Farsi with pure-tone thresholds ≤25 dB HL at audiometric frequencies from 0.25 to 8.00 kHz.Results: Reliability, the variability of test-retest score differences, was 1.28 dB in quiet and 1.08 dB in noise. Average speech reception thresholds in Quiet = 19.4 dB(A). Average signal/noise ratios at threshold were Noise Front = -5.4 dB, Noise Right = -13.7 dB, and Noise Left = -13.7.Conclusions: The reliability and norms for the Farsi HINT are comparable to those for other languages, enabling comparison of test results across languages.

Improvement in drought tolerance of lemon balm, Melissa officinalis L. under the pre-treatment of LED lighting.

Stress priming (pre-exposure of plants to various types of moderate stresses) could affect plant responses to subsequent severe stresses. Drought stress is one of the major threats to plants which reduces the global agricultural productions. Here we demonstrated that light emitting diodes (LEDs)-driven tolerant to drought stress in lemon balm plantlets was highly correlated with priming with these lighting sources. Plantlets of the two genotypes of M. officinalis L. were first grown in 4 incubators with different LED lamps, including white LEDs (380-760 nm), blue LEDs (460 nm), red LEDs (650 nm) and red + blue LEDs (70%:30%), in a greenhouse for 4 weeks. The potted plants were then subjected to drought stress. Under drought stress, LED-primed plants maintained significantly higher fresh and dry weight, relative water content (RWC), concentration of soluble sugars, antioxidant activity and higher content of proline, H2O2, abscisic acid (ABA) and rosmarinic acid than non-primed plants. The results of Real-Time RT-PCR confirmed that LED pretreatment up-regulated the expression levels of respiratory burst oxidase homologues (RBOHs) or NADPH oxidase, 9-cis epoxy carotenoid dioxygenase (NCED), and rosmarinic acid synthase (RAS), while down-regulated that of ABA 8'-hydroxylase (ABA8Ox). These findings suggest, for the first time, that pre-treatment of plants with red + blue LEDs could improve their growth and quality under drought stress.

Maternal Perception of Self-Efficacy and Involvement in Young Children with Prelingual Hearing Loss.

Appropriate intervention needs to support families and consider them as a part of rehabilitation program. Parents who have high self-efficacy are more likely to put their knowledge and skills into action and have positive interactions with their children. In addition, there has been a positive relation between parental involvement and child educational success. The aim of present study was evaluating maternal perception of both self-efficacy and involvement between mothers of children with hearing aid and cochlear implant via Scale of Parental Involvement and Self Efficacy (SPISE) and exploring relationship between maternal self-efficacy and parental involvement and child factors. 100 mothers of children with hearing loss were available. 49 mothers participated in study, filled SPISE, and return it on time. SPISE consisted of three sections (1) demographic information, (2) maternal self-efficacy, (3) parental involvement. All cases had received at least 6 months auditory training and speech therapy. Participants included 30 (61.2%) mothers of children with hearing aid, 19 (38.8%) mothers of children with cochlear implant. ANOVA analysis showed that there is no significant difference between hearing aid (HA) and cochlear implant (CI) groups in term of self-efficacy and parent-involvement except for question 21 (comfortable in participating in individualized program) that score in HA group was significantly higher than CI group. Results of present study has practical implications for early interventionists working with families. Every early intervention program should consider families to reach maximum outcome. Early interventionists can use SPISE to evaluate parental selfefficacy and involvement and work on parents with low score to achieve the best results.

Binaural sensitivity to temporal fine structure and lateralization ability in children with suspected (central) auditory processing disorder.

OBJECTIVE: Previous studies have shown that a subgroup of children with suspected (central) auditory processing disorder (SusCAPD) have insufficient ability to use binaural cues to benefit from spatial processing. Thus, they experience considerable listening difficulties in challenging auditory environments, such as classrooms. Some researchers have also indicated the probable role of binaural temporal fine structure (TFS) in the perceptual segregation of target signal from noise and hence in speech perception in noise. Therefore, in the present study, in order to further investigate the underlying reason for listening problems against background noise in this group of children, their performance was measured using binaural TFS sensitivity test (TFS-LF) as well as behavioral auditory lateralization in noise test, both of which are based on binaural temporal cues processing. METHODS: Participants in this analytical study included 91 children with normal hearing and no listening problems and 41 children (9-12 years old) with SusCAPD who found it challenging to understand speech in noise. Initially, the ability to use binaural TFS was measured at three frequencies (250, 500 and 750Hz) in both the groups, and the results of preliminary evaluations were compared between normal children and those with SusCAPD who participated in the study. Thereafter, the binaural performance of the 16 children with SusCAPD who had higher thresholds than the normal group at all three frequencies tested in TFS-LF test was examined using the lateralization test in 7 spatial locations. RESULTS: Total 16 of the 41 children with SusCAPD who participated in this study (39%) showed poor performance on the TFS-LF test at all three frequencies, compared to both normal children and other children in the APD group (p<0.05). Furthermore, children in the APD group with binaural TFS coding deficits at all three frequencies revealed significant differences in the lateralization test results compared to normal children (p<0.05). CONCLUSION: Findings of the current study demonstrated that one of the underlying causes for the difficulty understanding speech in noisy environments experienced by a subgroup of children with SusCAPD can be the reduced ability to benefit from binaural TFS information. This study also showed that a reduced ability to use binaural TFS cues in the group of children with SusCAPD was accompanied by reduced binaural processing abilities in the lateralization test which also admit the presence of binaural temporal processing deficits in this group of children.

Enhancing Working Memory Capacity in Persian Cochlear Implanted Children: A Clinical Trial Study.

INTRODUCTION: Sensory deprivations such as hearing impairment that affect sensory input have a secondary impact on cognitive functions such as working memory (WM). WM capacity is an important cognitive component that processes language-related activities. Moreover, several studies have shown a deficit in WM in children with a cochlear implant (CI). We aimed to assess the performance of children with CIs in pre- and post-training sessions and compare their scores on a battery of WM tests to investigate the efficacy of a WM training program. MATERIALS AND METHODS: Twenty-five children aged 7-10 years with a CI participated in this study. To train their WM, a computer game was used. In order to examine auditory WM, a test battery including standardized digit span (forward and backward variations), non-word and sentence repetition (subtest of the Test of Language Development-Primary) were assessed in pre- and post-training test sessions at Shiraz Implant Center. RESULTS: There were statistically significant differences between pre- and post-training test scores on all subtests. Test score differences were statistically significant for forward digit span (P=0.003), backward digit span (P=0.001), non-word repetition (P=0.001), and sentence repetition tasks (P=0.003) before and after training sessions. CONCLUSION: Training may enhance WM capacity. With regards to the importance of WM in literacy and learning, it seems applying such intervention programs may be helpful in the rehabilitation of implanted children.

Social Representation of "Loud Music" in Young Adults: A Cross-Cultural Study.

BACKGROUND: Exposure to recreational noise, particularly music exposure, is considered one of the biggest public health hazards of our time. Some important influencing factors such as socioeconomic status, educational background, and cross-cultural perspectives have previously been found to be associated with attitudes toward loud music and the use of hearing protection. Although culture seems to play an important role, there is relatively little known about how it influences perceptions regarding loud music exposure in young adults. PURPOSE: The present study was aimed to explore cross-cultural perceptions of and reactions to loud music in young adults (18-25 yr) using the theory of social representations. RESEARCH DESIGN: The study used a cross-sectional survey design. STUDY SAMPLE: The study sample included young adults (n = 534) from five different countries (India, Iran, Portugal, the United States, and the United Kingdom) who were recruited using convenience sampling. DATA COLLECTION AND ANALYSIS: Data were collected using a questionnaire. Data were analyzed using a content analysis, co-occurrence analysis, and also χ² analysis. RESULTS: Fairly equal numbers of positive and negative connotations (∼40%) were noted in all countries. However, the χ² analysis showed significant differences between the countries (most positive connotations were found in India and Iran, whereas the most negative connotations were found in the United Kingdom and Portugal) regarding the informants' perception of loud music. The co-occurrence analysis results generally indicate that the category "negative emotions and actions" occurred most frequently, immediately followed by the category "positive emotions and actions." The other most frequently occurring categories included "acoustics," "physical aliment," "location," and "ear and hearing problems." These six categories formed the central nodes of the social representation of loud music exposure in the global index. Although some similarities and differences were noted among the social representations toward loud music among countries, it is noteworthy that more similarities than differences were noted among countries. CONCLUSIONS: The study results suggest that "loud music" is perceived to have both positive and negative aspects within society and culture. We suggest that the health promotion strategies should focus on changing societal norms and regulations to be more effective in decreasing the noise- and/or music-induced auditory symptoms among young adults.

Story retelling skills in Persian speaking hearing-impaired children.

OBJECTIVES: Since the pragmatic skills of hearing-impaired Persian-speaking children have not yet been investigated particularly through story retelling, this study aimed to evaluate some pragmatic abilities of normal-hearing and hearing-impaired children using a story retelling test. METHODS: 15 normal-hearing and 15 profound hearing-impaired 7-year-old children were evaluated using the story retelling test with the content validity of 89%, construct validity of 85%, and reliability of 83%. Three macro structure criteria including topic maintenance, event sequencing, explicitness, and four macro structure criteria including referencing, conjunctive cohesion, syntax complexity, and utterance length were assessed. The test was performed with live voice in a quiet room where children were then asked to retell the story. The tasks of the children were recorded on a tape, transcribed, scored and analyzed. RESULTS: In the macro structure criteria, utterances of hearing-impaired students were less consistent, enough information was not given to listeners to have a full understanding of the subject, and the story events were less frequently expressed in a rational order than those of normal-hearing group (P < 0.0001). Regarding the macro structure criteria of the test, unlike the normal-hearing students who obtained high scores, hearing-impaired students failed to gain any scores on the items of this section. CONCLUSIONS: These results suggest that Hearing-impaired children were not able to use language as effectively as their hearing peers, and they utilized quite different pragmatic functions.

Social representation of "music" in young adults: a cross-cultural study.

OBJECTIVE: This study was aimed to explore perceptions of and reactions to music in young adults (18-25 years) using the theory of social representations (TSR). DESIGN: The study used a cross-sectional survey design and included participants from India, Iran, Portugal, USA and UK. Data were analysed using various qualitative and quantitative methods. STUDY SAMPLE: The study sample included 534 young adults. RESULTS: The Chi-square analysis showed significant differences between the countries regarding the informants' perception of music. The most positive connotations about music were found in the responses obtained from Iranian participants (82.2%), followed by Portuguese participants (80.6%), while the most negative connotations about music were found in the responses obtained from Indian participants (18.2%), followed by Iranian participants (7.3%). The participants' responses fell into 19 main categories based on their meaning; however, not all categories were found in all five countries. The co-occurrence analysis results generally indicate that the category "positive emotions or actions" was the most frequent category occurring in all five countries. CONCLUSIONS: The results indicate that music is generally considered to bring positive emotions for people within these societies, although a small percentage of responses indicate some negative consequences of music.

Auditory Brainstem Response Improvements in Hyperbillirubinemic Infants.

BACKGROUND AND OBJECTIVES: Hyperbillirubinemia in infants have been associated with neuronal damage including in the auditory system. Some researchers have suggested that the bilirubin-induced auditory neuronal damages may be temporary and reversible. This study was aimed at investigating the auditory neuropathy and reversibility of auditory abnormalities in hyperbillirubinemic infants. SUBJECTS AND METHODS: The study participants included 41 full term hyperbilirubinemic infants (mean age 39.24 days) with normal birth weight (3,200-3,700 grams) that admitted in hospital for hyperbillirubinemia and 39 normal infants (mean age 35.54 days) without any hyperbillirubinemia or other hearing loss risk factors for ruling out maturational changes. All infants in hyperbilirubinemic group had serum bilirubin level more than 20 milligram per deciliter and undergone one blood exchange transfusion. Hearing evaluation for each infant was conducted twice: the first one after hyperbilirubinemia treatment and before leaving hospital and the second one three months after the first hearing evaluation. Hearing evaluations included transient evoked otoacoustic emission (TEOAE) screening and auditory brainstem response (ABR) threshold tracing. RESULTS: The TEOAE and ABR results of control group and TEOAE results of the hyperbilirubinemic group did not change significantly from the first to the second evaluation. However, the ABR results of the hyperbilirubinemic group improved significantly from the first to the second assessment (p=0.025). CONCLUSIONS: The results suggest that the bilirubin induced auditory neuronal damage can be reversible over time so we suggest that infants with hyperbilirubinemia who fail the first hearing tests should be reevaluated after 3 months of treatment.

Social representation of "hearing loss": cross-cultural exploratory study in India, Iran, Portugal, and the UK.

BACKGROUND: Hearing loss is one of the most common chronic conditions in older adults. In audiology literature, several studies have examined the attitudes and behavior of people with hearing loss; however, not much is known about the manner in which society in general views and perceives hearing loss. This exploratory study was aimed at understanding the social representation of hearing loss (among the general public) in the countries of India, Iran, Portugal, and the UK. We also compared these social representations. MATERIALS AND METHODS: The study involved a cross-sectional design, and participants were recruited using the snowball sampling method. A total of 404 people from four countries participated in the study. Data were collected using a free-association task where participants were asked to produce up to five words or phrases that came to mind while thinking about hearing loss. In addition, they were also asked to indicate if each word they presented had positive, neutral, or negative associations in their view. Data were analyzed using various qualitative and quantitative methods. RESULTS: The most frequently occurring categories were: assessment and management; causes of hearing loss; communication difficulties; disability; hearing ability or disability; hearing instruments; negative mental state; the attitudes of others; and sound and acoustics of the environment. Some categories were reported with similar frequency in most countries (eg, causes of hearing loss, communication difficulties, and negative mental state), whereas others differed among countries. Participants in India reported significantly more positive and fewer negative associations when compared to participants from Iran, Portugal, and the UK. However, there was no statistical difference among neutral responses reported among these countries. Also, more differences were noted among these countries than similarities. CONCLUSION: These findings provide useful insights into the public perception of hearing loss that may prove useful in public education and counseling.

Social representation of hearing aids: cross-cultural study in India, Iran, Portugal, and the United Kingdom.

BACKGROUND: The current study was aimed at understanding the social representation of hearing aids in India, Iran, Portugal, and the United Kingdom. We also compared these results to explore the cross-cultural differences and similarities among these countries. METHODS: The study involved a cross-sectional design, and the data were collected from four different countries using the snowball sampling method. Data were analyzed using a content analysis to identify the most-similar categories of responses reported, a co-occurrences analysis to see which of these categories are reported commonly, and a chi-square analysis to study if there was any association between positive, neutral, and negative connotations among participants in different countries. RESULTS: The current study revealed four different social representations of hearing aids from India, Iran, Portugal, and the United Kingdom, and also a global index. CONCLUSION: The study results provide very useful insights into how hearing aids are represented in the society. These findings may have important implications for public education and also for manufacturers from the viewpoint of designing and marketing hearing aids in different countries.

Audiologists' preferences for patient-centredness: a cross-sectional questionnaire study of cross-cultural differences and similarities among professionals in Portugal, India and Iran.

OBJECTIVE: Patient-centredness has become an important aspect of health service delivery; however, there are a limited number of studies that focus on this concept in the domain of hearing healthcare. The objective of this study was to examine and compare audiologists' preferences for patient-centredness in Portugal, India and Iran. DESIGN: The study used a cross-sectional survey design with audiologists recruited from three different countries. PARTICIPANTS: A total of 191 fully-completed responses were included in the analysis (55 from Portugal, 78 from India and 58 from Iran). MAIN OUTCOME MEASURE: The Patient-Practitioner Orientation Scale (PPOS). RESULTS: PPOS mean scores suggest that audiologists have a preference for patient-centredness (ie, mean of 3.6 in a 5-point scale). However, marked differences were observed between specific PPOS items suggesting these preferences vary across clinical situations. A significant level of difference (p<0.001) was found between audiologists' preferences for patient-centredness in three countries. Audiologists in Portugal had a greater preference for patient-centredness when compared to audiologists in India and Iran, although no significant differences were found in terms of age and duration of experience among these sample populations. CONCLUSIONS: There are differences and similarities in audiologists' preferences for patient-centredness among countries. These findings may have implications for the training of professionals and also for clinical practice in terms of optimising hearing healthcare across countries.